from healthy volunteers
|
Cardiovascular risk correlates with [ADMA] |
|||||
|
Low risk group |
High risk group |
||||
|
Patient # |
[ADMA], mM |
|
Patient # |
[ADMA], mM |
|
|
LR01 |
0.062 |
|
HR01 |
0.573 |
|
|
LR02 |
0.112 |
|
HR02 |
0.573 |
|
|
LR03 |
0.115 |
|
HR03 |
0.599 |
|
|
LR04 |
0.137 |
|
HR04 |
0.603 |
|
|
LR05 |
0.162 |
|
HR05 |
0.639 |
|
|
LR06 |
0.175 |
|
HR06 |
0.733 |
|
|
LR07 |
0.180 |
|
HR07 |
1.009 |
|
|
LR08 |
0.192 |
|
HR08 |
1.104 |
|
|
LR09 |
0.200 |
|
HR09 |
1.202 |
|
|
LR10 |
0.205 |
|
HR10 |
1.643 |
|
|
LR11 |
0.210 |
|
|
|
|
|
LR12 |
0.227 |
|
|
|
|
|
LR13 |
0.242 |
|
|
|
|
|
LR14 |
0.247 |
|
|
|
|
|
LR15 |
0.252 |
|
|
|
|
|
LR16 |
0.260 |
|
|
|
|
|
LR17 |
0.292 |
|
|
|
|
|
LR18 |
0.367 |
|
|
|
|
|
LR19 |
0.417 |
|
|
|
|
|
LR20 |
0.444 |
|
|
|
|
|
Mean |
0.225 |
|
Mean |
0.868 |
|
|
Std. Dev. |
0.0979 |
|
Std. Dev. |
0.361 |
|
Venous blood from 30 volunteers was collected in tubes and centrifuged at 2500 x g to separate plasma. To determine asymmetric dimethylarginine (ADMA) concentrations, plasma samples were diluted 1:100 and 1:000 and analyzed with an Enzyme Linked Immunosorbant Assay (ELISA) kit, a gift from Cardiovasics Medical Science Laboratory, (Palo Alto, California). To classify as low or high-risk, plasma ADMA concentrations measured for each volunteer was tested on partially purified preparations of endothelium nitric oxide synthase (eNOS) (Reif and McCreedy).
